as my girlfriend started her new job as research associate she brought an Excel spreadsheet with the oligo data from her new lab with her. We transferred the 145 oligos into OligoChecker in just under 3 minutes using the Excel template. Her boss had designed some oligos to introduce mutations into a sequence so we ran the oligos against the sequence - they look fine EXCEPT THERE ARE NO MISMATCHES IN THE ALIGNMENT! The oligos her boss designed and bought are not mutagenic! Time saved before the mistake would have been discovered by sequencing the "mutated" plasmid: about a week. Money saved: about $1,000. :-)