EnzymeX - 3.1

The interface is wonderful and extremely intuitive. It is terrific, both functionally, and visually! Unfortunately, there are a few errors in the program. I emailed the authors, but received a form reply, which is disappointing.

Firstly, I cannot trust the recommended buffer combinations! For example, BamHI is listed in NEB 1, 2, 3 and 4 as 75%, 100%, 50% and 75% respectively. From the NEB website, activity is actually 75%, 100%, 100% and 100%.

Secondly, ORFs and restriction sites that span across the origin of circular vectors are not identified!

Unfortunately, while EnzymeX appears to function as expected otherwise, these two semi-major bugs makes me wary about relying too heavily on this program. From the authors' reply, it appears it is no longer in development. "Unfortunately Papers takes up most of our time these days, so it could take a while before this feature would appear." This is a pity, as these bugs should not be too difficult to fix.

the molecular weights given for the amino acids are each short the weight of water, i.e. their mass is calculated as if each amino acid was in a dehydrated form in a protein, not as the program's chemical structures show, which are in its normal, unpolymerized form.